Stephen Floor, PhD

Assistant Professor
Department of Cell & Tissue Biology
Research Overview: 

The Floor Lab studies the mechanisms by which RNA impacts human gene expression in health and disease. RNA chaperones are a major focus of the lab, which remodel RNA structures and RNA-protein interactions. We use deep sequencing, molecular biology of purified proteins, and imaging approaches to define the in vitro and cellular mechanisms of RNA chaperones. Many DEAD-box RNA chaperones are genetically altered in cancers and other diseases. We study the molecular basis of such alterations using cancer cell lines and genetically engineered stem cell models, aiming to better understand human biology and nominate new therapeutic targets or treatment regimens.

Primary Thematic Area: 
Cancer Biology & Cell Signaling
Secondary Thematic Area: 
Developmental & Stem Cell Biology
Research Summary: 
RNA-mediated control of gene expression in health and disease
Mentorship Development: 

4/12/19  Acknowleding and Negotiating the Mentee-Mentor Tensions Inherent in the Research Lab (Parnassus)

Websites

Publications: 

Aberrant cortical development is driven by impaired cell cycle and translational control in a DDX3X syndrome model.

eLife

Hoye ML, Calviello L, Poff AJ, Ejimogu NE, Newman CR, Montgomery MD, Ou J, Floor SN, Silver DL

SRSF1 governs progenitor-specific alternative splicing to maintain adult epithelial tissue homeostasis and renewal.

Developmental cell

Yu T, Cazares O, Tang AD, Kim HY, Wald T, Verma A, Liu Q, Barcellos-Hoff MH, Floor SN, Jung HS, Brooks AN, Klein OD

Directed evolution of the rRNA methylating enzyme Cfr reveals molecular basis of antibiotic resistance.

eLife

Tsai K, Stojkovic V, Noda-Garcia L, Young ID, Myasnikov AG, Kleinman J, Palla A, Floor SN, Frost A, Fraser JS, Tawfik DS, Fujimori DG

DDX3X and DDX3Y are redundant in protein synthesis.

RNA (New York, N.Y.)

Venkataramanan S, Gadek M, Calviello L, Wilkins K, Floor S