Stephen Floor, PhD

Assistant Professor
Department of Cell & Tissue Biology
Research Overview: 

The Floor Lab studies the mechanisms by which RNA impacts human gene expression in health and disease. RNA chaperones are a major focus of the lab, which remodel RNA structures and RNA-protein interactions. We use deep sequencing, molecular biology of purified proteins, and imaging approaches to define the in vitro and cellular mechanisms of RNA chaperones. Many DEAD-box RNA chaperones are genetically altered in cancers and other diseases. We study the molecular basis of such alterations using cancer cell lines and genetically engineered stem cell models, aiming to better understand human biology and nominate new therapeutic targets or treatment regimens.

Primary Thematic Area: 
Cancer Biology & Cell Signaling
Secondary Thematic Area: 
Developmental & Stem Cell Biology
Research Summary: 
RNA-mediated control of gene expression in health and disease

Websites

Publications: 

PTBP1 mRNA isoforms and regulation of their translation.

RNA (New York, N.Y.)

Arake de Tacca LM, Pulos-Holmes MC, Floor SN, Cate JHD

The Translation Inhibitor Rocaglamide Targets a Bimolecular Cavity between eIF4A and Polypurine RNA.

Molecular cell

Iwasaki S, Iwasaki W, Takahashi M, Sakamoto A, Watanabe C, Shichino Y, Floor SN, Fujiwara K, Mito M, Dodo K, Sodeoka M, Imataka H, Honma T, Fukuzawa K, Ito T, Ingolia NT

CRISPR-Cas9 genome editing in human cells occurs via the Fanconi anemia pathway.

Nature genetics

Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schäfer AJ, Floor SN, Corn JE

The Traffic Jam: Polyamine Prevalence Pauses Protein Production.

Molecular cell

Venkataramanan S, Floor SN