Markus Delling, PhD

Asst Professor
+1 415 476-2308
Research Overview: 

About four billion years ago, while earth was covered with a primordial soup enriched with the building blocks of life, the equal occurrence of chiral molecules (non-superimposable “left-handed” vs “right-handed” molecules) fell out of balance. As a consequence, today’s life is asymmetric.

In humans, our visceral organs such as heart, pancreas, and intestine are asymmetrically positioned along the left-right (L-R) axis. Essential key players in establishing L-R asymmetry are fluid flow, primary cilia, ion channels and the embryonic node. The key sensory organelle in L-R patterning is the primary cilium, a hair-like structure protruding from the plasma membrane of most mammalian cells and believed to function like an antenna. Early during development primary cilia utilize ciliary ion channels and electric signaling to “sense” directed fluid flow and orchestrate asymmetric gene expression and ultimately L-R patterning. The fundamental molecular mechanisms of how primary cilia sense their local environment, including the movement of fluids, are only poorly understood. Yet a plethora of severe human diseases such congenital heart disease, autosomal dominant polycystic kidney disease (ADPKD), obesity, and mental retardation can be attributed to improper cilia function.

My lab studies the molecular mechanisms of how primary cilia utilize ion channels and GPCRs to sense their local environment and control such important processes as left-right asymmetry formation. Major research goals in our lab include

  1. Identify the environmental signals that activate ciliary Ca2+ channels
  2. Understand cilia-dependent signaling cascades governed by electric signaling.
  3. Define the electric signaling within the embryonic node during early steps of establishing asymmetry.  
  4. Identify small molecule agonists and antagonists of ciliary ion channels as novel therapeutics for the treatment of ciliopathies such as ADPKD
  5. Understand the diversity of primary cilia signaling with respect to ion channel composition.

We use a variety of different approaches including mouse genetics, RNA sequencing, cutting edge primary cilia Ca2+ imaging, electrophysiology and biochemistry.

Primary Thematic Area: 
Cancer Biology & Cell Signaling
Secondary Thematic Area: 
Developmental & Stem Cell Biology
Research Summary: 
Calcium signaling in primary cilia during development and disease



ADPKD-Causing Missense Variants in Polycystin-1 Disrupt Cell Surface Localization or Polycystin Channel Function.

bioRxiv : the preprint server for biology

Ha K, Loeb GB, Park M, Pinedo A, Park CH, Brandes N, Ritu F, Ye CJ, Reiter JF, Delling M

The Concise Guide to PHARMACOLOGY 2023/24: Ion channels.

British journal of pharmacology

Alexander SPH, Mathie AA, Peters JA, Veale EL, Striessnig J, Kelly E, Armstrong JF, Faccenda E, Harding SD, Davies JA, Aldrich RW, Attali B, Baggetta AM, Becirovic E, Biel M, Bill RM, Caceres AI, Catterall WA, Conner AC, Davies P, De Clerq K, Delling M, Di Virgilio F, Falzoni S, Fenske S, Fortuny-Gomez A, Fountain S, George C, Goldstein SAN, Grimm C, Grissmer S, Ha K, Hammelmann V, Hanukoglu I, Hu M, Ijzerman AP, Jabba SV, Jarvis M, Jensen AA, Jordt SE, Kaczmarek LK, Kellenberger S, Kennedy C, King B, Kitchen P, Liu Q, Lynch JW, Meades J, Mehlfeld V, Nicke A, Offermanns S, Perez-Reyes E, Plant LD, Rash L, Ren D, Salman MM, Sieghart W, Sivilotti LG, Smart TG, Snutch TP, Tian J, Trimmer JS, Van den Eynde C, Vriens J, Wei AD, Winn BT, Wulff H, Xu H, Yang F, Fang W, Yue L, Zhang X, Zhu M

Testing the ion-current model for flagellar length sensing and IFT regulation.


Ishikawa H, Moore J, Diener DR, Delling M, Marshall WF

Conversion of anterograde into retrograde trains is an intrinsic property of intraflagellar transport.

Current biology : CB

Nievergelt AP, Zykov I, Diener D, Chhatre A, Buchholz TO, Delling M, Diez S, Jug F, ?tep?nek L, Pigino G