Margaret Feeney, MD

Department of Pediatrics
Research Overview: 

The Feeney Lab studies the human T cell response to infection and age-based differences in the immune response during gestation and infancy. The overarching goal of our research is to better understand how the human infant responds to infection in order to inform the development of vaccines and immune-based therapies.

Our main interest is human immunity to malaria caused by P. falciparum, a pathogen that has co-evolved with humans for millenia. Projects currently underway include the identification of key antigenic targets during liver-stage P. falciparum infection, interactions between innate and adaptive lymphocytes, and the role of immunoregulation in subverting the development of protective immunity and following malaria infection or vaccination. We use samples and data from cohorts of women, infants, and children to characterize the antimalarial immune response using high parameter technologies (scRNAseq, TCRseq, flow/mass/spectral cytometry), and collaborate with leading malaria epidemiologists to correlate immune parameters with clinical outcomes in well-characterized cohorts.

A longstanding interest of the lab is the development of immune function during early life. Fetal and infant T cells are functionally distinct from those of adults. This is in part due to the tolerogenic in utero environment, but in addition there are intrinsic differences in the programming of fetal lymphocytes that shape their response to pathogens. A better understanding of these differences could have major translational implications for the design of vaccines and adjuvants that maximize immunogenicity in the neonate. In our lab, we study how T cells respond to pathogen-derived antigens encountered in utero or during early infancy (e.g. placental malaria, congenital CMV infection). We have established a large biobank of umbilical cord blood cells and longitudinal follow-up samples to facilitate the study of infant immune responses at the cellular and molecular level. A major current emphasis in the lab is the role of gamma delta (gd) T cells, a unique population of semi-innate cells that play a key role in immunity in the fetus and infant. A subset of gd T cells exhibit intrinsic reactivity to malaria metabolites and correlate with vaccine protection, while a distinct subset has been shown to contribute to immunity to CMV. Our lab is working to understand how these critical early effector cells can be harnessed to mitigate infection during infancy.

To facilitate these studies, we have developed robust international collaborations, primarily in Uganda, where we have developed significant laboratory infrastructure to study the human immune response to malaria in a highly endemic setting. The Feeney Lab is committed to training scientists from historically under-represented backgrounds, in both the U.S. and abroad. 

Primary Thematic Area: 
Secondary Thematic Area: 
Virology & Microbial Pathogenesis
Research Summary: 
Human immunity to malaria, T cell immunity in the fetus and infant



Malaria-specific Type 1 regulatory T cells are more abundant in first pregnancies and associated with placental malaria.


Kirosingh AS, Delmastro A, Kakuru A, van der Ploeg K, Bhattacharya S, Press KD, Ty M, Parte L, Kizza J, Muhindo M, Devachanne S, Gamain B, Nankya F, Musinguzi K, Rosenthal PJ, Feeney ME, Kamya M, Dorsey G, Jagannathan P

Highly potent, naturally acquired human monoclonal antibodies against Pfs48/45 block Plasmodium falciparum transmission to mosquitoes.


Fabra-Garc?a A, Hailemariam S, de Jong RM, Janssen K, Teelen K, van de Vegte-Bolmer M, van Gemert GJ, Ivanochko D, Semesi A, McLeod B, Vos MW, de Bruijni MHC, Bolscher JM, Szabat M, Vogt S, Kraft L, Duncan S, Kamya MR, Feeney ME, Jagannathan P, Greenhouse B, Dechering KJ, Sauerwein RW, King CR, MacGill RS, Bousema T, Julien JP, Jore MM

Potent transmission-blocking monoclonal antibodies from naturally exposed individuals target a conserved epitope on Plasmodium falciparum Pfs230.


Ivanochko D, Fabra-Garc?a A, Teelen K, van de Vegte-Bolmer M, van Gemert GJ, Newton J, Semesi A, de Bruijni M, Bolscher J, Ramjith J, Szabat M, Vogt S, Kraft L, Duncan S, Lee SM, Kamya MR, Feeney ME, Jagannathan P, Greenhouse B, Sauerwein RW, Richter King C, MacGill RS, Bousema T, Jore MM, Julien JP

Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria.

Science translational medicine

Ty M, Sun S, Callaway PC, Rek J, Press KD, van der Ploeg K, Nideffer J, Hu Z, Klemm S, Greenleaf W, Donato M, Tukwasibwe S, Arinaitwe E, Nankya F, Musinguzi K, Andrew D, de la Parte L, Mori DM, Lewis SN, Takahashi S, Rodriguez-Barraquer I, Greenhouse B, Blish C, Utz PJ, Khatri P, Dorsey G, Kamya M, Boyle M, Feeney M, Ssewanyana I, Jagannathan P

In Utero Activation of NK Cells in Congenital CMV Infection.

The Journal of infectious diseases

Vaaben AV, Levan J, Nguyen CBT, Callaway PC, Prahl M, Warrier L, Nankya F, Musinguzi K, Kakuru A, Muhindo MK, Dorsey G, Kamya MR, Feeney ME