Aimee Kao, MD, PhD

Associate Professor in Res
Department of Neurology
Research Overview: 

Despite intensive research efforts, many of the basic mechanisms underlying neurodegenerative diseases remain unclear.  We are tackling this problem by focusing on two genes linked to neurodegenerative disease, progranulin and tau.  By taking advantage of the genetic tractability of C. elegans and the ability to model disease in a dish with patient-derived induced pluriopotent stem cells (iPSCs), we are pursuing studies to understand how mutant forms of these proteins alter fundamental cellular processes such as endocytic trafficking, protein degradation, programmed cell death and stress response.

Progranulin and regulation of programmed cell death kinetics: Disease-related progranulin mutations result in a genetic haploinsufficiency, or production of progranulin protein levels that are approximately half that of normal.  Using the nematode C. elegans and cultured murine cells, we recently discovered that loss of progranulin affects the rate of clearance of apoptotic cells.  One project in the lab is to better understand how progranulin regulates programmed cell death kinetics and how progranulin deficiency leads to neurodegeneration.  Future work will involve understanding the effects of progranulin on microglial function and interrogating the relative roles for granulin versus progranulin.

Pathophysiological Implications of the MAPT A152T mutation: Mutations in the MAPT or tau gene have been associated with several neurodegenerative diseases and tau "tangles" are found in the brains of those afflicated with Alzheimer's Disease.  Recently, a polymorphism in MAPT at position 152 has been described.  We are characterizing the effect of this variant C. elegans and iPSC-derived neurons by observing behavioral alterations as well as biochemical characteristics of tau such as post-translational modification and protein-protein interactions.

Identification of novel regulators of programmed cell death: The core machinery of programmed cell death pathways were first identified in C. elegans. We have recently identified new players in apoptosis as well as new functions for previously identified cell death genes.  Our future work will be to better characterize these cell death effectors, and place them in context of established cellular metabolism.

Primary Thematic Area: 
Secondary Thematic Area: 
Developmental & Stem Cell Biology
Research Summary: 
Programmed Cell Death and the Pathophysiology of Neurodegenerative Diseases
Mentorship Development: 

4/26/19    Sharpening your Mentoring Skills (SyMS) with Sharon Milgram (Mission Bay)    
11/23/20    Building Community in the UCSF MSTP 
9/11/20    Mentoring Across Differences
2/18/21    Three Truths and Three Tries: Facing and Overcoming Critical Social Justice Challenges at the Micro, Mezzo, and Macro Levels    



Phenotypic Screening Using High-Content Imaging to Identify Lysosomal pH Modulators in a Neuronal Cell Model.

ACS chemical neuroscience

Chin MY, Ang KH, Davies J, Alquezar C, Garda VG, Rooney B, Leng K, Kampmann M, Arkin MR, Kao AW

TSC1 loss increases risk for tauopathy by inducing tau acetylation and preventing tau clearance via chaperone-mediated autophagy.

Science advances

Alquezar C, Schoch KM, Geier EG, Ramos EM, Scrivo A, Li KH, Argouarch AR, Mlynarski EE, Dombroski B, DeTure M, Dickson DW, Yokoyama JS, Cuervo AM, Burlingame AL, Schellenberg GD, Miller TM, Miller BL, Kao AW

Processing of progranulin into granulins involves multiple lysosomal proteases and is affected in frontotemporal lobar degeneration.

Molecular neurodegeneration

Mohan S, Sampognaro PJ, Argouarch AR, Maynard JC, Welch M, Patwardhan A, Courtney EC, Zhang J, Mason A, Li KH, Huang EJ, Seeley WW, Miller BL, Burlingame A, Jacobson MP, Kao AW

Genetically Encoded, pH-Sensitive mTFP1 Biosensor for Probing Lysosomal pH.

ACS sensors

Chin MY, Patwardhan AR, Ang KH, Wang AL, Alquezar C, Welch M, Nguyen PT, Grabe M, Molofsky AV, Arkin MR, Kao AW