Stephen Floor, PhD

Assistant Professor
Department of Cell & Tissue Biology
stephen.floor@ucsf.edu

The Floor Lab studies the mechanisms by which RNA impacts human gene expression in health and disease. RNA chaperones are a major focus of the lab, which remodel RNA structures and RNA-protein interactions. We use deep sequencing, molecular biology of purified proteins, and imaging approaches to define the in vitro and cellular mechanisms of RNA chaperones. Many DEAD-box RNA chaperones are genetically altered in cancers and other diseases. We study the molecular basis of such alterations using cancer cell lines and genetically engineered stem cell models, aiming to better understand human biology and nominate new therapeutic targets or treatment regimens.

Primary Thematic Area: 
Cancer Biology & Cell Signaling
Secondary Thematic Area: 
Developmental & Stem Cell Biology
Research Summary: 
RNA-mediated control of gene expression in health and disease

Websites

Publications: 

Widespread translational remodeling during human neuronal differentiation

bioRxiv preprint

John D Blair, Dirk Hockemeyer, Jennifer A Doudna, Helen S Bateup, Stephen N Floor

CRISPR-Cas9 Genome Editing In Human Cells Works Via The Fanconi Anemia Pathway

bioRxiv preprint

Chris D. Richardson, Katelynn R. Kazane, Sharon J. Feng, Nicholas L. Bray, Axel J. Schaefer, Stephen Floor, Jacob Corn

Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes.

Nature biotechnology

Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA

Medulloblastoma-associated DDX3 variant selectively alters the translational response to stress.

Oncotarget

Oh S, Flynn RA, Floor SN, Purzner J, Martin L, Do BT, Schubert S, Vaka D, Morrissy S, Li Y, Kool M, Hovestadt V, Jones DT, Northcott PA, Risch T, Warnatz HJ, Yaspo ML, Adams CM, Leib RD, Breese M, Marra MA, Malkin D, Lichter P, Doudna JA, Pfister SM, Taylor MD, Chang HY, Cho YJ