Kevin Shannon, MD

Professor
Department of Pediatrics
Department of Cellular and Molecular Pharmacology
shannonk@peds.ucsf.edu

The Shannon Lab exploited inherited predispositions and recurring cytogenetic alterations as entry points to search for genetic lesions that contribute to leukemogenesis. This work has converged on the Ras signaling pathway and on the role of chromosome 7 deletions (monosomy 7) in leukemogenesis. We uncovered mutations in the NF1 and PTPN11 genes in juvenile myelomonocytic leukemia (JMML) and other myeloid malignancies. NF1, which encodes a GTPase activating protein for Ras, functions as a tumor suppressor gene. The PTPN11 and gene encodes SHP-2, a non-receptor protein tyrosine phosphatase that relays signals from activated growth factor receptors to Ras and other effectors. Based on these human data, we harnessed the interferon-inducible Mx1-Cre recombinase to develop tractable mouse models of human myeloprolfierative neoplasms by inactivating Nf1 or by expressing oncogenic Nras and Kas in hematopoietic cells. To model more aggressive, multi-step human cancers, we performed retroviral insertional mutagenesis in Nf1, Nras, and Kras mutant mice to generate acute myeloid leukemia (AML and T lineage acute lymphoblastic leukemia (T-ALL). We extensively investigated inhibitor of MEK (PD0325901) and PI3 kinase (GDC-0941 in these models and have used them to elucidate mechanisms of response and resistance in vivo. We are also exploring novel strategies for selectively inhibiting the biochemical output of oncogenic N-Ras. The long-term goal of this research is to develop rational therapeutic strategies for preventing drug resistance and relapse in AML and other advanced hematologic cancers. We have also used chromosome engineering to model segmental deletions of chromosome band 7q22 found in myeloid malignancies and to interrogate how these alterations perturb hematopoiesis and contribute to leukemia.

Primary Thematic Area: 
Cancer Biology & Cell Signaling
Secondary Thematic Area: 
Human Genetics
Research Summary: 
Aberrant Ras Signaling in Development and Cancer

Websites

Publications: 

Stat5 is critical for the development and maintenance of myeloproliferative neoplasm initiated by Nf1 deficiency.

Haematologica

Sachs Z, Been RA, DeCoursin KJ, Nguyen HT, Mohd Hassan NA, Noble-Orcutt KE, Eckfeldt CE, Pomeroy EJ, Diaz-Flores E, Geurts JL, Diers MD, Hasz DE, Morgan KJ, MacMillan ML, Shannon KM, Largaespada DA, Wiesner SM

KRAS insertion mutations are oncogenic and exhibit distinct functional properties.

Nature communications

White Y, Bagchi A, Van Ziffle J, Inguva A, Bollag G, Zhang C, Carias H, Dickens D, Loh M, Shannon K, Firestone AJ

KRAS Engages AGO2 to Enhance Cellular Transformation.

Cell reports

Shankar S, Pitchiaya S, Malik R, Kothari V, Hosono Y, Yocum AK, Gundlapalli H, White Y, Firestone A, Cao X, Dhanasekaran SM, Stuckey JA, Bollag G, Shannon K, Walter NG, Kumar-Sinha C, Chinnaiyan AM

Functional evidence implicating chromosome 7q22 haploinsufficiency in myelodysplastic syndrome pathogenesis.

eLife

Wong JC, Weinfurtner KM, Alzamora Mdel P, Kogan SC, Burgess MR, Zhang Y, Nakitandwe J, Ma J, Cheng J, Chen SC, Ho TT, Flach J, Reynaud D, Passegué E, Downing JR, Shannon K