David Toczyski, PhD

Professor in Residence
Cancer Research Institute
toczyski@cc.ucsf.edu

The DNA damage response is a critical responder to environmental stress. We are using a combination of candidate approaches and phosphoproteomics to identify targets of the DNA damage response pathway that alter cellular physiology in response to DNA damage. These targets include regulators of origin firing, transcription, mitosis and histone modifications. 

Ubiquitination rivals phosphorylation in its widespread importance in many processes. Degradation, vesicular transport and the DNA damage response are all regulated by ubiquitination. Our lab has a long-standing interest in two ubiquitin ligases, the APC and the SCF. Both have important roles in cell cycle progression, as well as other processes. We have developed methods to identify substrates of these ligases, and are applying these to both yeast and human cells.

Primary Thematic Area: 
Cancer Biology & Cell Signaling
Secondary Thematic Area: 
None
Research Summary: 
Activation of the DNA damage checkpoint

Websites

Publications: 

Parallel Parkin: Cdc20 Takes a New Partner.

Molecular cell

Meza-Gutierrez F, Hundley FV, Toczyski DP

Prb1 Protease Activity Is Required for Its Recognition by the F-Box Protein Saf1.

Biochemistry

Mark KG, Meza-Gutierrez F, Johnson JR, Newton BW, Krogan NJ, Toczyski DP

DNA Damage Regulates Translation through ß-TRCP Targeting of CReP.

PLoS genetics

Loveless TB, Topacio BR, Vashisht AA, Galaang S, Ulrich KM, Young BD, Wohlschlegel JA, Toczyski DP