Biomedical Sciences Graduate Program | University of California, San Francisco

Daniel Bikle, MD, PhD
Regulation of cellular differentiation and function by calciotropic hormones

Selected Publications | Complete Publications

phone	(415) 221-4810, ext. 3338
email	Daniel.Bikle@ucsf.edu
additional websites	Cancer Center
secondary research affiliation	Tissue/Organ Biology & Endocrinology

My research is focused on the hormonal regulation of calcium metabolism, and includes projects involving the classic target tissues of bone and intestine as well as non classic tissues including the skin. One project attempts to understand the molecular mechanisms by which 1,25(OH)₂ D regulates intestinal calcium transport, in particular calcium transport across the brush border. Using an intestinal cell line, Caco2, we are overexpressing or blocking the expression of selected brush border proteins to determine whether such manipulations alter vitamin D regulated calcium transport in these cells. The second project is evaluating the effects of parathyroid hormone and insulin like growth factor-1 on bone formation, with particular interest in the effects of mechanical loading or unloading on the anabolic actions of these hormones, examining at the molecular level the signaling pathways mediating these hormones as affected by loading. The third project is examining the mechanisms by which calcium and 1,25(OH)2D regulate keratinocyte differentiation. We are focusing on the calcium receptor and the vitamin D receptor, and the signaling pathways and transcriptional events mediated by their activation.

Selected Publications

1. Bikle D, Teichert A, Hawker N, Xie Z, and Oda Y. 2007. Sequential regulation of keratinocyte differentiation by 1,25(OH)₂ D₃, VDR, and its coactivators. J Ster Biochem Molec Biol 103:396-404

2. Hawker N, Pennypacker S, Chang S, Bikle D. 2007.Regulation of human epidermal keratinocyte differentiation by the vitamin D receptor and its coactivators DRIP205, SRC2, and SRC3. J Invest Dermatol 127:874-880

3. Tu C-L, Chang W, Bikle DD. 2007.The role of the calcium sensing receptor in regulating intracellular calcium handling in human epidermal keratinocytes. J Invest Dermatol 127:1074-1083

4. Schauber J, Dorschner RA, Coda AB, Buchau AS, Liu PT, Kiken D, Helfrich YR, Kang S, Elalieh HZ, Steinmeyer A, Zugel U, Bikle DD, Modlin RL, Gallo RL.2007. Injury enhances TLR2 function and antimicrobial peptide expression through a vitamin D dependent mechanism. J Clin Invest 117:803-811

5. Oda Y, Ishikawa MH, Hawker NP, Yun Q-C, and Bikle DD. 2007. Differential role of two VDR coactivators, DRIP205 and SRC-3, in keratinocyte proliferation and differentiation. J Ster Biochem Mol Biol 103:776-780

6. Xie Z and Bikle DD 2007 The recruitment of phosphatidylinositol 3-kinase to the E-cadherin/catenin complex at the plasma membrane is required for calcium-induced phospholipase C- g 1 activation and human keratinocyte differentiation. J Biol Chem 282:8695-8703

7. Boudignon B, Bikle D, Kurimoto P, Elalieh H, Nishida S, Wang Y, Burghardt A, Majumdar S, Orwoll B, Rosen C, Halloran B. 2007. Insulin-like Growth Factor-I Stimulates Recovery of Bone Lost after a Period of Skeletal Unloading. J Appl Physiol 103:125-131

8. Dvorak MM, Chen T-H, Orwoll B, Garvey C, Chang W, Bikle DD, Shoback DM 2007. Constitutive activity of the osteoblast Ca ²⁺ -sensing receptor promotes loss of cancellous bone Endocrinology 148:3156-3163

9. Wang Y, Nishida S, Boudignon BM, Burghardt A, Elalieh HZ, Hamilton MM, Majumdar S, Halloran BP, Clemens TL, Bikle DD 2007. The IGF-I receptor is required for the anabolic actions of parathyroid hormone on bone. J Bone Min Res 22:1329-1337.

10. Schauber J, Oda U, Buchau AS, Steinmeyer A, Zugel U, Bikle DD, Gallo RL 2008 Histone acetylation in keratinocytes enables control of the expression of cathelicidin and CD14 by 1,25 dihydroxyvitamin D3. J invest Dermatol 128:816-824

11. Tu C-L, Chang W, Xie Z, and Bikle DD 2008 Inactivation of the calcium sensing receptor inhibits E-cadherin-mediated cell-cell adhesion and calcium-induced differentiation in human epidermal keratinocytes. J Biol Chem 283:3519-3528

information last updated June 2008