My lab and I specialize in the study of the spatio-temporal dynamics of immune responses, largely using imaging approaches. We focus upon molecular mechanisms that coordinate T cell biology as well as upon cellular dynamics that result in information-sharing amongst cells of the response in vivo. In the early 2000s we described the dynamics of cluster coalescence at the immunological synapse and have since identified key Myosin motors driving cellular motility towards synapses (mid 2000s). These remain a focus of the lab as we unravel the mechanisms that control T cell movement and establishment and movement of signaling clusters. We have also described the roles of costimulation and chemokines in modulating these behaviors. In the mid-2000s, we custom-built 2-photon microscopes and related biological tools for analyzing T cell function in situ. Concurrently, we are exploiting new models of human breast cancer and human lung disease to provide insight into the 4-dimensional coordination of the immune system.
Gérard, A., Khan, O., Beemiller, P., Oswald, E., Hu, J., Matloubian, M., Krummel, M.F. 2013. Secondary T cell-T cell synaptic interactions drive the differentiation of protective CD8+ T cells. Nat Immunol. 2013 Mar 10. doi: 10.1038/ni.2547. [Epub ahead of print]
Gérard, A., Beemiller, P., Friedman, R.S., Jacobelli, J., Krummel, M.F. 2013. Evolving immune circuits are generated by flexible, motile, and sequential immunological synapses. Immunol Rev. 2013 Jan;251(1):80-96. doi: 10.1111/imr.12021.
Cheng, L.E., Hartmann, K., Roers, A., Krummel, M.F., Locksley, R.M. 2013. Perivascular Mast Cells Dynamically Probe Cutaneous Blood Vessels to Capture Immunoglobulin E. Immunity. Jan 24;38(1):166-75. doi: 10.1016/j.immuni.2012.09.022. Epub 2013 Jan 3. [Epub ahead of print]
Zhang, Y., Chen, Y.C., Krummel, M.F., Rosen, S.D. 2012. Autotaxin through Lysophosphatidic Acid Stimulates Polarization, Motility, and Transendothelial Migration of Naive T Cells. J Immunol. Sep 7. [Epub ahead of print]
Beemiller, P., Jacobelli, J., Krummel, M.F. 2012. Integration of Signaling Microclusters Movement with Cellular Motility in Immunological Synapses. Nature Immunology, Nat Immunol. Jul 1. doi: 10.1038/ni.2364.
Thornton, E.E., Krummel, M.F., Looney, M.R. 2012. Live Imaging of the Lung. Curr Protoc Cytom. Apr;Chapter 12:Unit12.28.
Thornton, E.E., Looney M.R., Bose, O,. Sen, D., Sheppard, D., Locksley, R., Huang, X., Krummel, M.F. 2012. Spatiotemporally Separated Antigen Uptake by Alveolar Dendritic Cells and Airway Presentation to T Cells in the Lung. J Exp Med., 209(6):1183-99.
Engelhardt, J.J., Boldajipour, B., Beemiller, P., Pandurangi, P., Sorensen, C., Werb, Z., Egeblad, M., Krummel, M.F. 2012. Marginating Dendritic Cells of the Tumor Microenvironment Cross-Present Tumor Antigens and Stably Engage Tumor-Specific T Cells. Cancer Cell 21, March 20; 402-417.
Gilden, J.K., Peck, S., Chen, Y.C.M., Krummel, M.F. 2012. The septin cytoskeleton facilitates membrane retraction during motility and blebbing. J Cell Biol. Jan 9;196(1):103-14.
Khan, O., Headley, M., Gerard, A., Wei, W., Liu, L., Krummel, M.F. 2011. Regulation of T Cell Priming by Lymphoid Stroma. PLoS ONE 6(11): e26138. doi:10.1371/journal.pone.0026138.
Looney, M.R., Thornton, E.E., Sen, D., Lamm, W.J., Glenny, R.W., Krummel, M.F. 2010. Stabilized imaging of immune surveillance in the mouse lung. Nat Methods. 8(1):91-6.