Multiple intercellular contacts give rise to the complex systems behavior of the immune system. We are extending our imaging systems in order to study interactions amongst a variety of T cell partners such as dendritic cells and macrophages. Of particular interest are the multipartite interactions in which three or more cells come together simultaneously or sequentially to create complex biology. Novel biological insights can be gleaned by understanding the nature of cooperative and competitive cellular interactions. In addition, we are using molecular genetics coupled with imaging to identify novel molecular players recruited to immune cell-cell interfaces. Our ultimate goal is to uncover the methods by which the immune system regulates the decision between tolerance and immunity
Jacobelli,J., Bennett, F.C., Pandurangi, P. and Krummel, M.F. 2009. Myosin-IIA and ICAM-1 Regulate the Interchange between Two Distinct Modes of T cell Migration J. Immunol.82: 2041-50
Tooley, A.J., Gilden, J., Jacobelli, J., Trimble, W, Kinoshita, M. and Krummel, M.F. 2008. The Septin Cytoskeleton is Essential for Cortical Integrity and Persistent Motility in Amoeboid T lymphocytes. Nature Cell Biology. Nov 30. [Epub ahead of print]
Sabatos, C.A., Doh, J. Chakravarti, S. Friedman, R.S., Pandurangi, P.G., Tooley, A.J. Krummel, M.F. 2008. A Synaptic Basis for Paracrine Interleukin-2 Signaling in Activating T cells. Immunity.29(3): 238-248.
Krummel, M.F. and Macara I. 2006. M aintenance and modulation of T cell polarity Nature Immunology. 7, 1143-49.
Friedman, R.S. Jacobelli, J, and Krummel, M.F. 2006. Su rface-bound Chemokines Capture and Prime T cells For Synapse Formation. Nature Immunology 7 , 1101-8.
Tang, Q., Adams, J.Y., Tooley, A.J., Bi, M., Serra, P., Santamaria, P., Krummel, M.F.* and Bluestone, J.A.* (*co-last authors) 2006. Visualizing regulatory T cell control of autoimmune response in NOD diabetic mice. Nature Immunology, 7, 83-92
Boisvert, J., Edmondson, S. and Krummel, M.F. 2004. Immunological Synapse Formation Licenses CD40-CD40L Accumulations at T-APC Contact Sites. J. Immunol. 173, 3647-3652.
Jacobelli, J. Chmura, S.A., Buxton,D.B., Davis , M.M. and Krummel, M. F. 2004. A single class II myosin modulates T cell motility and stopping, but not synapse assembly.Nature Immunology5, 531 - 538
Moss,W.C., Irvine,D.C, Davis,M.M., and Krummel, M.F. 2002. Quantifying Signaling-Induced Reorientation of Cell Membrane and TCRs During Immunological Synapse Formation. PNAS. 99 15024-15029.
Krummel, M.F. , Sjaastad,M.D., Wülfing,C., and Davis, M.M. 2000. Differential clustering of CD4 and CD3 z during T cell recognition. Science. 289, 1349-1352.